With the development of recombinant DNA technology, scientific researchers can construct recombinant fusion proteins which express target gene and have an epitope tag. Therefore, epitope tags are frequently used for identification and purification of recombinant proteins. Epitope tag antibodies are highly specific monoclonal/polyclonal antibodies with commonly used dye and enzyme conjugates and can serve as a universal detection method in Western blot.
A monoclonal antibody specifically recognizes a single epitope within an antigen, thus having following advantages compared with polyclonal antibodies, low cross-reactivity, high specificity, excellent lot-to-lot stability.
Secondary antibodies can bind to antibodies, namely ‘anti-antibody’. Secondary antibodies can be used for indirect detection of the target antigens, and it can improve the sensitivity by amplifying signal and provide more flexibility for labeling and detection.
Polyclonal antibody is a group of immunoglobulin secreted by plasma cells in the organism, which occurred during the immune reaction when the body is stimulated by heterologous antigens (macromolecular antigen, hapten conjugates). Polyclonal antibodies have been widely applied in science research and diagnosis due to the various advantages, such as recognizing multiple antigen epitopes, causing precipitation reaction, short preparation time and low cost.
When a reaction occurs in a cell, it receives signal transmitted from outside the cell, and then the cell responds according to the information it received. The whole procedure from receiving external signals from cell receptors to making a comprehensive response is not only a signal transduction process, but also a process of gradually amplifying external signals, which involves a series of comprehensive responses.
Highly specific & sensitive monoclonal antibody Complete immunohistochemical reagents Detailed guide to the IHC experiment Accurate and stable results
Acetylation is related with protein activity, therefore the activity of target protein can be reflected through the specific identification of acetylated target protein by acetylated antibodies, and to study the function and regulation of protein.
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